Home > Browse Issues > Vol.40 No.1
The Effect of Egr1 on C2C12 Differentiation
Cui Yafeng, YanYunqin, Li Shufeng, Tong Huili*
The Laboratory of Cell and Dvelepomental Biology, College of Life Science,
Northeast Agricultural University, Harbin 150030, China
Abstract: Egr1 (early growth response protein 1)， as a transcription factor， plays important roles in many biological processes， such as cell proliferation， differentiation， apoptosis and so on. However， its effect on C2C12 cells differentiation remains unclear. Western blot and immunofluorescence assays were used to determine the protein level and localization of Egr1 in C2C12 during its differentiation. To explore its effect on C2C12 differentiation， Egr1 expression was activated and inhibited using the CRISPR/Cas9 technique. Egr1 protein level reached the peak on the 5th day of differentiation and then gradually decreased during C2C12 differentiation. Egr1 localized in the cytoplasm and nucleus of C2C12 cells. Egr1 level gradually increased when C2C12 induced to differentiation. During the C2C12 differentiation， myotube fusion rate， myogenin (MYOG) and myosin heavy chain (MYH2) levels increased or decreased significantly when Egr1 were up-regulated or down-regulated， respectively. In summary， the results showed that Egr1 promotes C2C12 differentiation in vitro.
CN
EN