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Genetic Toxicity of Alginate-derived Oligosaccharide on Vicia faba Root Tip Cells
Ning Bu, Lian-Ju Ma*, Xiao-Lu Jiang1, Sheng-Hou Wang
College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China; 1Division of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China
Abstract: The effects of different concentrations of alginate-derived oligosaccharide (ADO) on Vicia faba root tip cells were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index， the micronucleus rate and chromosomal aberration rate of Vivia faba root tip cells induced by cyclophosphamide (CP) under different concentrations of ADO. The result showed that ADO could effectively inhibit the micronucleus rate. During concentration of 10-0.625 g/L， the number of micronucleus of Vivia faba root tip cells were reduced with decrease concentration of ADO， but it was increased when concentration of ADO was lower than 0.625 g/L. The mitotic index was increased， while chromosomal aberration rate were lowed under all concentration of ADO. Turkey test showed all chromosomal aberration rate were significantly different from control (P<0.05). So， ADO has an obvious effect on restraining chromosomal aberration and increasing division of cell of Vivia faba root tip.
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