Application of iTRAQ Technology in Animal Production

Abstract: In recent years， the rapid development of proteomics has led to rapid advance in related experimental techniques. Compared with other technologies， isobaric tags for relative and absolute quantifcation (iTRAQ) technology is widely accepted by the majority of scholars with its high-throughput， high sensitivity and high repeat ability， which allows it to be employed in a variety of complex samples for relative and absolute quantitative research. It has become one of the main means of proteome quantitative research when it is combined with multi-dimensional liquid chromatography and tandem mass spectrometry technologies. With the continuous optimization of iTRAQ technology， it has been widely used in animals， microorganisms and other fields. This paper mainly covers the basic principles， advantages and disadvantages of iTRAQ technology and its application in animal production， and mainly summarizes the following contents. The impact of iTRAQ technology in beef muscle development and quality are analyzed. The differential analysis of milk protein in dairy cows and the screening of defense protein for mastitis are introduced and the meat performance， hair follicle development and intestinal immunity of sheep are illustrated. This paper goes over briefly the development of skeletal muscle of pig， the defense of porcine circo virus type 2 (PCV2)， and the analysis of the influencing factors of frozen semen are included. The muscle development of broilers is elaborated and the research progress of eggshell quality and the defense mechanism of infectious bursal disease (IBD) are analyzed， in order to provide the basis for the scientific research and production practice of animal husbandry.

CSTR: 32200.14.cjcb.2017.12.0012