miR-638 Inhibits the Proliferation and Tubal Formation of Ewing Sarcoma Cells Partially through Regulating the VEGFA

Abstract: This work was to investigate the level of miR-638 in Ewing sarcoma cells (EWS) and its effects on the proliferation and tube formation of EWS， so as to further identify the putative mechanisms involved. The level of miR-638 was detected in EWS comparing with the mesenchymal stem cells (MSC) by qRT-PCR assays. We restored the level of miR-638 in RD-ES and SK-ES-1 cells through transfection with artificial miR-638 mimic. The CCK-8 and tubule formation experiments were used to explore the effects of miR-638 on the proliferation and tubal formation of EWS cells， respectively. Then qRT-PCR and Western blot assays were performed to explore the effects of miR-638 on the level of VEGFA. Rescue assays were performed to identify whether VEGFA was involved in miR-638-mediated suppressive effects on EWS. The levels of miR-638 in three Ewing sarcoma cell lines were significantly lower than those in MSC (P<0.05); Compared with the control groups， the proliferation rates and the number of branches in the formation were significantly decreased (P<0.05). qRT-PCR and Western blot analysis showed that over-expression of miR-638 could significantly inhibit the levels of mRNA and protein of VEGFA. The results of rescue showed that the level of miR-638 mimic and pcDNA-empty target gene VEGFA was the lowest， the growth rate was the slowest， and the number of branches was the least. miR-638 was suppressed in EWS cells， and its over-expression inhibited cell proliferation and tubal formation of EWS， which might be mediated targeting VEGFA partially.

CSTR: 32200.14.cjcb.2017.12.0011