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Bioinformatics Analyses of Exosome from REV-Infected DF-1 Cells
Wang Xiaoman1,2#, Zhuang Pingping1#, Sun Xia3, Du Xusheng1, Wang Xiaoyu1, Cheng Ziqiang1,2, Wang Guihua1,2*
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China; 2Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai’an 271018, China; 3Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China
Abstract: The study was to identify the differentially expressed proteins and microRNA (miRNA)， their gene function and involved signal pathway of exosome from REV-infected DF-1 cells. The differential proteins and miRNA of exosome were screened between the virus infection group and control group by proteomics and transcription detection technology， and then analyzed with GO function enrichment and KEGG signal pathway database. The results demonstrated that 101 different expressed proteins were screened with 56 up-regulated and 45 down-regulated. These proteins were involved in 155 signal pathways， and the number of proteins involved in the cancer pathway was the highest. There was 3 up-regulated miRNA screened. Both the target proteins (integrins) of miRNA-155 and miRNA-146a-3p， and the differential protein actinrelated 2/3 complexs (Arp2/3) participated in actin cytoskeleton signal pathway. Otherwise， they contained viral components， and involved in cell signaling， immunization， adhesion， locomotion， biological regulation and other processes. These results suggested that the differentially expressed proteins and miRNA， their gene function and involved signal pathway of exosome from REV infected DF-1 cells were associated with tumor formation. Exosome pathway may play an important role in the REV tumorigenesis mechanism.
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