Study on the Regularity and Temporal Correlation of Mast Cell Aggregation and Degranulation in Rat Model of Pulmonary Hypertension in Left Heart Disease

Abstract: The article is to investigate the regularity and temporal correlation of mast cell aggregation and degranulation in rat model of pulmonary hypertension in left heart disease (PH-LHD). 66 male minor Sprague- Dawley (SD) rats were randomly divided into control group (n=6)， sham operation group (n=6) and model group (n=54). The rats in the control group entered the experimental procedure immediately without any treatment， and the rats in the sham operation group entered the experimental procedure after 64 days of sham operation， and the rats in model group entered the experimental procedure (6 rats each) on 1， 3， 5， 8， 15， 22， 36， 50 and 64 d， respectively， after the successful of banding of the ascending aorta. The lung tissue was fixed， cut and made into pathological sections to observe the aggregation and degranulation of mast cells peripheral pulmonary vessels. Obvious mast cell agregation was observed since 36 d in perivascular area of pulmonary arterioles with diameter 20-50 μm and 50- 100 μm， and most cells were degranulated. The increased density of mast cells in whole lung tissue were observed. Mast cell agregation was observed since 50 d in perivascular area of pulmonary arterioles with diameter >100 μm， and most cells were granulated. Mast cells played a crucial role in PH-LHD rat model. The mast cells around the pulmonary arteries with diameter >100 μm had little effect on the formation of pulmonary hypertension， but the mast cells around 20-50 μm and 50-100 μm significantly involved.

CSTR: 32200.14.cjcb.2017.12.0007