Genetic Polymorphisms of Exon 1 of Hormone Sensitive Lipase Gene in Pigs

Abstract: Single-nucleotide polymorphisms of exon1 of hormone sensitive lipase (HSL) gene were tested using PCR-SSCP. Correlations between the HSL gene and the birth weight， the weaning weight， the weight at the six-month and the backfat thickness were analyzed. On the basis of the published DNA sequence (AJ000483) of the porcine HSL gene， five pairs of primers were designed， and one polymorphism was found in the PCR product amplified with P5 primers. A G→A transition at 874 site of exon1 of HSL gene was detected by sequencing the homozygotes. Three genotypes existed. The results show that: firstly， the genotype distribution wasn't in consistent among the different pig breeds. Significant differences (P<0.01) were found for the genotype distribution while the Landrace and the Large White compared with the Laiwu and the Yimeng pig breeds; whereas no significant differences(P>0.05) existed while the Landrace compared with the Large White， the Laiwu compared with the Yimeng pig breeds. Secondly， on the basis of the fixed effect model， significant differences (P<0.05) were found in the backfat thickness， whereas no significant differences (P>0.05) existed in the birth weight， the weaning weight and the weight at the six-month. Thirdly， using least square analysis， it was shown that individuals of the BB genotype have significantly lower (P<0.05) backfat thickness than those of AB and AA genotypes， with the order being BB    

CSTR: 32200.14.cjcb.2007.05.0027