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Display of Human Survivin on Surface of Escherichia coli Using N-terminal Domain of Ice Nucleation Protein
Luo Yanxi1, Jiang Yili2, Yan Hui1*
1Institute of Material Medical, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China; 2Medical School, Hangzhou Normal University, Hangzhou 310013, China
Abstract: Survivin is a highly specific broad-spectrum tumor-associated antigens. The feasibility of displaying human survivin protein on the surface of Escherichia coli (E. coli) cells was studied using an ice nucleation protein (INP) surface display system. The survivin gene fragment and the Cherry reporter gene were fused to the N-terminus of INP， and the surface displayed vector pET-INP-CHY-SUV was constructed and transformed into E. coli BL21 (DE3). Red fluorescence of recombinant bacteria can be detected after being induced by IPTG， and the intensity decreased by digestion of trypsin. The whole-cell ELISA and immune fluorescence assay showed the green fluorescence. The above results suggested that human survivin was expressed in recombinant bacteria and successfully displayed on the cell surface and lay the foundation for further development of new tumor DNA vaccine.
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