The Effects and Mechanism Studies of Recombinant Protein rLj-112 on Proliferation， Migration and Apoptosis of Human Non-small Cell Lung Cancer A549 Cells

Abstract: We studied the ability of rLj-112 protein from the Lamprey Japanica on anti-tumor of A549 cells and its regulatory mechanism in this study. We used MTT assay to detect cell proliferation. Giemsa staining assay and Hoechst 33258 staining were employed to examine cell apoptosis of A549 cells after Lj-112 treated. Western blot was used to examine cell apoptosis related signal proteins. It was found that after treated with rLj-112， the proliferation of A549 cells was inhibited and IC50 value was 1.64 μmol/L. The inhibition effect is in a dosedependent manner. The migration assay showed that after treated with different concentrations of rLj-112 proteins the inhibition effect was in a dose-dependent manner. The same situation occurred in the invasion test. The Hoechst 33258 staining experiment showed rLj-112 could induce the apoptosis of A549 cell. The Western blot analysis showed that cleaved-caspase3 protein and cleaved-PARP protein were improved in A549 cells treated with rLj-112 and demonstrated that rLj-112 induced apoptosis of A549 cells through caspase3/PARP pathway. The levels of p-AKT， p-PI3K and p-Erk1/2 were decreased in the rLj-112 treated A549 cells， demonstrated that rLj-112 reduced AKT/PI3K pathway in A549 cells.

CSTR: 32200.14.cjcb.2017.12.0003