Generation of Induced Pluripotent Stem Cells without Integration of Genetic Materials Mediated with Vector

Li Nan#, Guo Hongmei^#, Wang Mingyu, Ling Wenhui, Qiu Xiaoyan, Li Yuemin, Liu Xulei, Deng Yanan, Xiao Xiong^*

College of Animal Science and Technology, Southwest University, Chongqing 400715, China

Abstract: Induced pluripotent stem cells (iPSCs) have become one of the research focuses in medicine， biology， and so on， because they have the characteristics of “self-renewal” and differentiation which are similar to embryonic stem cells (ESCs). But the generation of iPSCs mainly depended on the expression of transcription factors mediated with retrovirus or lentivirus-based vector， these iPSCs bear certain risks， such as tumorigenicity， which resulted from the integration of exogenous genetic materials and viral vector into genome of recipient cells. At the same time， lower efficiency of reprogramming mediated with substance， such as recombinant proteins or small molecules was observed. Therefore， some vectors and methods were successfully developed to generate iPSCs without insertional transgene expression. This paper reviewed the researches about the applications of gene expression vector systems including Cre/loxP recombination system， piggyBac transposition system， oriP/EBNA1 episomal vector system and minicircle DNA vector system on the generation of iPSCs to provide a theoretical and technological reference for safely clinical applications of iPSCs.

CSTR: 32200.14.cjcb.2017.11.0017