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U83 box C/D snoRNA as a Molecular Marker in the Phylogeny of Drosophilidae
Ling Xu1,2, Yu-Ping Luo1, Dong-Gen Zhou1, Si-Guang Li1*
1College of Life Science, Nanchang University, Nanchang 330031, China; 2College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
Abstract: Based on FlyBase and U83 in Drosophila melanogaster， ten new U83 gene homologues were identified in Drosophilidae which were located in intron of rpl3 respectively. The homologous sequences were compared， and the molecular trees were reconstructed by neighbor-joining method of PAUP4.0 program using Anopheles gambiae as outgroup. The result reflected the evolutionary relationship in Drosophilidae roughly. Sequences were enlarged to the entire U83 located introns to get more information， and the phylogenetic tree constructed by those sequences was more correct compared with the former one. Genes of box C/D snoRNA were used for constructing phylogenetic tree for the first time， our study confirmed the good effect of U83 box C/D snoRNA as a molecular marker in the phylogenetic analysis of Drosophilidae.
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