Home > Browse Issues > Vol.39 No.11
Effects of Neural Stem Cells on Treating Hypoxic-Ischemic Encephalopathy through P25 and P35 Proteins
Tian Yuguang1, Pang Wei1, An Lei2, Wei Wei3, Gu Weiwang1, Wang Yujue1, Yue Min1*
1Department of Laboratory Animal Center, Southern Medical University, Guangzhou 510515, China; 2Department of the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China; 3Department of Guangdong Cord Blood Bank, Guangzhou 510000, China
Abstract: The objective of this study is to evaluate the efficacy on neurological functional recovery after transplantation of neural stem cells (NSCs) and the changes of P25 and P35 proteins in adult rats. 60 rats (7-day-old Sprague-Dawley) were randomly assigned to sham-operated group (n=20)， ischemia group (n=20) and NSCs transplanted group (n=20). Rotarod test and Morris test were performed at 5， 7， 14， 21 days after treatment to measure the behaviors of the rats. Hematoxylin-eosin staining was used to observe the pathological changes of brain damage in neonatal rats. Western blot was used to analyse the levels of P35 and P25 in hippocampus. The immunofluorescence double dye was used to verify the levels of P35 and P25 proteins. Rotarod test result showed
that motor function recovered gradually and restored to near the normal level on the 21th day in NSCs group and there was a significant difference between NSCs group and HIE group on the 14th day (P<0.05). Morris test showed that escape latency significantly shortened in NSCs group at each time points compared with HIE group (P<0.05). Pathological results showed that hippocampal neuron edema， nuclear chromatin structure is not clear， and vacuoles appeared in HIE group. Compared with HIE group， pathological changes in brain were significantly lessened. Western blot showed that the changes of P35 and P25 levels were significantly different in other groups compared with the Sham group. The P35 level decreased while P25 level increased in HIE group (P<0.05).
Compared with the HIE group， the P35 levels increased while P25 level decreased in NSCs group (P<0.05). The levels of P35 and P25 protein were verified by immunofluorescence double dye in cytological level. Our results showed that NSCs promoted the recovery of neurological function in rats after ischemic brain injury possibly by up regulating P35 level expression and decreasing the expression of P25 protein level.
that motor function recovered gradually and restored to near the normal level on the 21th day in NSCs group and there was a significant difference between NSCs group and HIE group on the 14th day (P<0.05). Morris test showed that escape latency significantly shortened in NSCs group at each time points compared with HIE group (P<0.05). Pathological results showed that hippocampal neuron edema， nuclear chromatin structure is not clear， and vacuoles appeared in HIE group. Compared with HIE group， pathological changes in brain were significantly lessened. Western blot showed that the changes of P35 and P25 levels were significantly different in other groups compared with the Sham group. The P35 level decreased while P25 level increased in HIE group (P<0.05).
Compared with the HIE group， the P35 levels increased while P25 level decreased in NSCs group (P<0.05). The levels of P35 and P25 protein were verified by immunofluorescence double dye in cytological level. Our results showed that NSCs promoted the recovery of neurological function in rats after ischemic brain injury possibly by up regulating P35 level expression and decreasing the expression of P25 protein level.
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