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LPS-induced BLP Tolerance and the Role of Actin Cytoskeleton in THP-1 Cell Line
Jian-Cang Liu, Rong Zhou, Liang-Ming Liu, Ren Liu, Nan Xiao*
State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Research Institute of Surgery, Daping Hospital, the Third Military Medical University, Chongqing 400042, China
Abstract: It is remain uncertain whether bacterial lipopolysaccharide (LPS)-toleranced monocyte cross-toleranced with bacterial lipoprotein (BLP). The study was aimed to observe the LPS tolerance and BLP cross-tolerance induced by LPS (10 ng/ml) and the changes of actin， and to explore the role of actin cytoskeleton in LPS-tolerance and BLP cross-tolerance in THP-1. The human monocyte cell line (THP-1) were adopted to establish the LPS-toleranced and BLP cross-toleranced cellular models. Actin skeleton， supernatant concentration of cytokins (TNF-α， IL-1β and IL-6) and NF-κB DNA binding activity was tested. When THP-1 were insulted with LPS (100 ng/ml) or BLP (100 ng/ml)， The cellular morphologic changes were significant with the formation of pseudopod and the actin reorganized to form the actin-band， NF-κB DNA binding activity upregulated in nuclear， and the concentration of cytokins (TNF-α， IL-1β and IL-6) in supernatant increased in THP-1. Pretreatment THP-1 with LPS (10 ng/ml)， the morphology， cytokines and NF-κB DNA binding activity were improved. Phalladin， a specifical actin cytoskeleton reorganization inhibitor， party abolished the LPS tolerance and BLP cross-tolerance in THP-1 cell induced by 10 ng/ml LPS. The results suggested that LPS (100 ng/ml) and BLP (100 ng/ml) could trigger the reorganization of actin cytoskeleton， activate NF-κB transcription， and increase the cytokins (TNF-α， IL-1β and IL-6) releasing， which were closely associated with the activatation of inflammation. LPS (10 ng/ml) pretreatment could induce LPS tolerance and BLP cross-tolerance in THP-1 which was at least partly associated with actin cytoskeleton.
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