Home > Browse Issues > Vol.39 No.9
Effects of Overexpression of DLX1 on Migration， Invasion， Apoptosis and Proliferation in Osteosarcoma Cell MG63
Lü Fengxiang1, Kang Quan2, Qiu Chao1, Dong Zixing1, Luo Qing1*
1Department of Pediatric Research Institution, Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of pediatrics, Chongqing 400014, China; 2Department of Hepatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China
Abstract: To investigate the effects of overexpression of DLX1 (distal-less homeobox 1) on migration， invasion， apoptosis and proliferation of osteosarcoma cell MG63， we infected the MG63 cells with AdDLX1 (adenovirus distal-less homeobox 1) and AdRFP (adenovirus red fluorescence protein). Then the expression level of DLX1 was confirmed by RT-PCR and Western blot. The cell migration and invasion were analyzed by Transwell assay. DAPI staining and flow cytometry were used to detect the cell apoptosis. The cell proliferation was detected
by CCK-8 assay. The expression of β-catenin， a crucial molecule in Wnt/β-catenin pathway， was determined by RT-PCR and Western blot. The results showed that the mRNA and protein levels of DLX1 were siginificantly increased after infection with AdDLX1， and the capacities of cell migration and invasion were down-regulated. Overexpression of DLX1 could not change the apoptosis and proliferation of MG63 cells. The expression of β-catenin mRNA and protein levels were up-regulated in MG63 cells. These results suggested that the Wnt/β-catenin pathway which involved in the migration and invasion of MG63 cells was inhibited by DLX1.
by CCK-8 assay. The expression of β-catenin， a crucial molecule in Wnt/β-catenin pathway， was determined by RT-PCR and Western blot. The results showed that the mRNA and protein levels of DLX1 were siginificantly increased after infection with AdDLX1， and the capacities of cell migration and invasion were down-regulated. Overexpression of DLX1 could not change the apoptosis and proliferation of MG63 cells. The expression of β-catenin mRNA and protein levels were up-regulated in MG63 cells. These results suggested that the Wnt/β-catenin pathway which involved in the migration and invasion of MG63 cells was inhibited by DLX1.
CN
EN