The Effect of the Nanoporous Alumina Films with Different Pore Diameters on the Cell Migration

Abstract: To investigate the effects of the nanoporous anodic alumina (NAA) films with different pore diameters on the Hela cells migration， the Scanning Electron Microscope (SEM) and the Energy Dispersive X-ray Spectroscopy were used to characterize the microcosmic structure and chemical components of the NAA films respectively. The Hela cells were cultured in confocal dishes， divided into three groups (control group， 40-70 nm pore diameter group and 200-300 nm pore diameter group)， to perform wound healing assay， randomly picked several observing points to observe 24 hours continuously in the live cell imaging system. The cell migration analysis was performed in terms of migration area， migration distance of the edge and migration speed of single cell. The growth status of the cells after cultured 24 hours on both films was characterized by the SEM. The results showed that the 40-70 nm pore diameter group was able to speed the cell migration compared to the control group (P<0.05)， while the 200-300 nm pore diameter group was slower (P<0.05). These data suggested that the NAA films with defferent pore sizes had different effects on the cell migration， which might provide the evidence for the design， study and development of the materials controlling cell behavior in the clinical research.

CSTR: 32200.14.cjcb.2017.08.0012