Effect of ANLN on Cell Migration and Invasion of Liver Cancer Cells and Its Potential Mechanism

Abstract: The aim of this study was to investigate the effect of actin-binding protein (ANLN) on migration and invasion in hepatocellular carcinoma. The mRNA levels of ANLN in 20 paired hepatocellular carcinoma (HCC) tissues and adjacent nontumoral tissues were examined by qRT-PCR. Knockdown of ANLN gene was performed by lentivirus-mediated RNA interference and the efficiency was analyzed by qRT-PCR and Western blot analysis. Migration assay and invasion assay were carried out to examine the effect of ANLN knockdown on cell migration and invasion. The mRNA and protein level of matrix metallopeptidase 9 (MMP9) were analyzed by qRT-PCR and Western blot analysis， respectively. Our results showed that the mRNA level of ANLN was significantly upregulated in HCC tissues compared with adjacent tissues in 20 cases. The ANLN mRNA level was significantly associated with migration and invasion. Lentivirus-mediated shRNA interference inhibited markedly both ANLN mRNA and protein levels. Knockdown of ANLN inhibited Huh-7 cell migration and invasion. Furthermore， knockdown of ANLN gene inhibited MMP9 mRNA and protein level. MMP9 overexpression significantly abolished inhibitory effect of ANLN knockdown on cell migration and invasion. Taken together， these data indicated that ANLN knockdown inhibited migration and invasion of HCC cell by regulating MMP9.

CSTR: 32200.14.cjcb.2017.08.0009