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Primary Study on the Regulation of Pluripotent Factor Expression of BT549 Cells with Long Non-Coding RNA Z38
Sun Yuanyuan1, Liu Bin1, Ouyang Jing1, Mo Linbo1, Tang Zhengshan1, Qiao Xiaoyin1, Li Fangjun2, Yang Yinke1*
1College of Biology, Hunan University, Changsha 410082, China; 2Department of Social Medicine, Hunan Province People’s Hospital, Changsha 410002, China
Abstract: Z38 is a newly discovered long non-coding RNA that promotes the formation of breast cancer， but the specific mechanism is unclear. The aim of this study was to investigate the effect of Z38 on the expression of pluripotency gene NANOG， SOX2 and OCT4 in BT549 cells. The correlation between Z38 and CD44+CD24–/low subpopulations was analyzed by flow cytometry and RT-qPCR (Real-time quantitative polymerase chain reaction). Z38-shRNA was used to interfere Z38 level in BT549 cells. The mRNA and protein levels of NANOG， SOX2 and OCT4 were detected by RT-qPCR and Western blot. The results showed that the expression levels of Z38 and CD44+CD24–/low subpopulations were different in MCF-7， MDA-MB-453 and BT549 breast cancer cells. The ratio of CD44+CD24–/low subpopulations in BT549 cells was relatively high， and the percentage of CD44+CD24–/low subgroups decreased in BT549 cells after interference of Z38. The results also showed that the genes NANOG and SOX2 were down-regulated at mRNA and protein levels， while the gene OCT4 had no significant change in mRNA and protein level. Interference of Z38 affected the expression of BT549 pluripotency factor， especially downregulating the NANOG and SOX2 genes closely related to the differentiation of breast cancer stem cells， suggesting that it may be involved in regulating the differentiation of breast cancer stem cells.
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