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Glutamine Activates PI3K/Akt Signaling Pathway to Promote Replication of Vaccinia Virus
Xu Wanbin1, Wang Jiaojiao1*, Chen Jianhua2, Zhou Xiumei1, Wang Ruwei3, Fang Ling3, Yan Hui4
1Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310008, China; 2Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, China; 3Zhejiang Conba Pharmaceutical Co., Ltd, Hangzhou 310018, China; 4Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
Abstract: Glutamine (Gln) plays an important role in cell growth and metabolism. It can activate phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway. A variety of viruses can maintain cell survival through the PI3K/Akt signaling pathway， and inhibit cell apoptosis and maintain the virus infection. This study mainly investigated the role of Gln in the process of vaccinia virus strain western reserve (WR) infection and its potential mechanism. The protein levels of PI3K/Akt signaling pathway were measured by Western blot. The cell positive rate was determined by flow cytometry. The WR titer was assessed by crystal violet dyeing test. The mRNA levels of gene E3L and A46R were determined by qPCR (quantitative Real-time PCR). The results showed that Gln significantly promoted the levels of proteins in PI3K/Akt signaling pathway. Compared with normal Gln group， PI3K/Akt signaling pathway inhibitors significantly reduced the cell positive rate， the titer and the mRNA levels of E3L and A46R (P<0.05). The results showed that glutamine could promote the WR replication by activating PI3K/Akt signaling pathway in A549 cell.
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