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The Effect of Exdogenous Direct Current Electric Fields on Migration of Lung Adenocarcinoma Cell Line Calu-3
Li Sha1,2, Wang Xiaoyan1,2, Zhao Sanjun1,2, Gao Runchi1,2, Zhao Min1,3, Shi Limin1,2*
1School of Life Sciences, Yunnan Normal University, Kunming 650500, China;2Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, China; 3School of Medicine, University of California at Davis, California 95616, USA
Abstract: We analyzed the effect of direct current electric fields (dc EFs) on lung adenocarcinoma cells Calu-3 through Time-lapse microscopy. The cells without electric field were taken as the control group. The cells of experimental group were exposed to dc EFs with intensity of 2 V/cm， 4 V/cm and 6 V/cm， respectively. The cells of Calu-3 migrated through two way， including isolated cells and clustered cells. Our data showed that dc EFs had important effect on the migrations of Calu-3. Both isolated cells and clustered cells of Calu-3 moved randomly in the absence of an EF. In the presence of dc EFs with different strengths， both the two type cells migrated toward the cathode electrode of electric field. The parameters of migration ability， including directedness index， persistence index， track speed and displacement speed had significant differences between control and experimental groups. Generally， the dc EFs mad Calu-3 cells possess more obvious directional ability and faster speed. Both isolated and clustered cells achieved maximal electrotaxis in 4 V/cm EFs. The clustered cells of Calu-3 exhibited greater sensitivity to dc EFs but ran more slowly than the isolated cells under EFs with same strength.
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