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Preliminary Establishment of Cell Lines Derived from Different Tissues of Amur Sturgeon， Acipenser schrencki Brandt
Yan Meng1, Yan Zhang3, Lin Zhang1,2, Han-Bing Xiao1, Luo-Xin Li3, Yan-Qing Yang1, Ling-Bing Zeng1,2,3*
1Key Laboratory of Freshwater Ecology and Healthy Aquaculture, Chinese Academy of Fishery Sciences; 2Fish Pathology Laboratory, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences; 3Key Laborator
Abstract: By using the tissue explantation technique， primary culture of cells derived from the liver， spleen， kidney， fin and heart tissue of Amur sturgeon， Acipenser schrencki Brandt， were conducted. In 2-3 days cells migrate out around tissues and in 10 days post culture cells outgrow to monolayer. Continuous cell lines from liver， spleen， kidney and heart tissues were established after subculture of primary cells with trypsin-EDTA digestion method. The optimized conditions for these cells are: minimum essential medium eagle抯 (MEME)， 25 ℃ 20% fetal bovine serum. Cells were cryopreserved with preservative DMSO in liquid nitrogen and grew well after recovery. The establishment of cell lines derived from Amur sturgeon has provided important experimental materials for the research on sturgeon virosis and genetic resources conservation.
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