The Application of Intravital Staining Microtechnic and Green Fluorescent Protein in the Experimental Course for Undergraduates

Abstract: The principle and usage of laser scanning confocal microscope are very important content in cell biology experimental course for undergraduates. In the current teaching procedures， green fresh leaves are used as material to observe the auto-fluorescent of chloroplast. The auto-fluorescent signal of chloroplast is strong and extensive， which leads to the result that the students cannot understand the specific fluorescent signal clearly. In this paper， the author stained the root tip of p35S::Naa10-GFP transformed Arabidopsis with PI (propidium iodide) and DAPI (4′，6-diamidino-2-phenylindole) and collected different fluorescent signal under relative wave length laser. Specific fluorescent signals merged images were obtained by using computer aided imaging technique. The design of this experiment is simple and the resulted images are clear and easy to observe， which make the beginners understand the principle and usage of laser scanning confocal microscope easily and deeply. The method introduced in this paper can be generalized in undergraduate experimental course in colleges and provides technical reference to study subcellular localization of a protein.

CSTR: 32200.14.cjcb.2017.07.0011