The Study of the Expression of Pyruvate Kinase M2 Gene in the Endometrium Mouse during the Early Stage of Pregnant

Abstract: The expression rules of the type of pyruvate kinase M2 (PKM2) gene in the endometrium of mouse during the early stage of pregnant were analyzed in this study. By establishing the pregnant model， we collected the endometrium of pregnant mice on D1， D4， D5， D6， D7 and implantation site and inter-implantation site on D5. By establishing the pseudopregnant model， we collected the endometrium of pseudopregnant mice on PD1， PD4， PD5， PD6， PD7. Real-time PCR and Western blot were employed to detect the expressions of PKM2. Immunohistochemistry was used to detect the distribution of PKM2 protein in the endometrium of mice at implantation site and inter implantation site on D5. The results revealed that the level of PKM2 mRNA in the endometrium of pregnant miceraised from D5， and reached the peak on D6， declined slightly on D7. The level of PKM2 protein in the endometrium of mice pregnant raised from D6， declined slightly on D7. The level of PKM2 protein on D6 and D7 had obvious differences compared with D1. In pseudopregnant model， the level of PKM2 mRNA in the endometrium of mice raised from PD6， consistented with PD6 and PD7. The level of PKM2 mRNA of PD6 and PD7 have obvious differences compared with PD1. There was no significant difference of PKM2 protein expression in pseudopregnant model. The level of PKM2 mRNA and protein in the endometrium of mice at implantation site was obviously higher than inter-implantation site on D5. Our results preliminarily revealed the expression rules of PKM2 gene in the endometrium， also provided an important clue to explore the mechanism how the PKM2 gene maintained the normal functions in the endometrium of mouse during the early stage of pregnant.

CSTR: 32200.14.cjcb.2017.07.0002