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Protein Arginine Methyltransferase 1 Promotes the Abilities of Proliferation and Migration in Pancreatic Cancer Cells
Wei Hong1#, Zhang Youli1#, Wang Jue1, Zhou Meng1, He Junbo1, Zhou Hailang1, Wang Dawei1, Zhou Gai1, Feng Wen1, Gong Aihua2*, Xu Min1*
1Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China;
2School of Medicine, Jiangsu University, Zhenjiang 212000, China
2School of Medicine, Jiangsu University, Zhenjiang 212000, China
Abstract: The aim of the present study is to investigate the effects of protein arginine methyltransferase 1 (PRMT1) on cell proliferation and migration in pancreatic cancer cells. We analyzed the PRMT1 gene expression in pancreatic non-tumor tissues and pancreatic cancer tissues of few databases. We then measured the levels of PRMT1 mRNA and protein in pancreatic cancer cells by Real-time PCR and Western blot. We knocked down PRMT1 in PaTu8988 and BxPC3 cells and detected the abilities of proliferation and migration by CCK-8 assay， colony-forming assay and migration assay. Epithelial-mesenchymal transition (EMT) markers were examined by Western blot.Conversely， we overexpressed PRMT1 in SW1990 cells and conducted the above-mentioned experiments again. The results showed that PRMT1 gene expression was higher in pancreatic cancer tissues than that in pancreatic non-tumor tissues at both mRNA and protein levels. The data in vitro revealed that PRMT1 knockdown inhibited the abilities of proliferation and migration， while PRMT1 overexpression promoted the above behaviors in pancreatic cancer cells.Further studies indicated that PRMT1 knockdown remarkably decreased the level of mesenchymal marker N-cadherin，and increased the level of epithelial marker E-cadherin. Conversely， PRMT1 overexpression resulted in the opposite effects. Our work suggested that PRMT1 promoted the ability of proliferation and migration in pancreatic cancer cells， and might provide a new therapeutic target for the clinical treatment of pancreatic cancer.
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