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Effect of Mitochondrial DNA Haplogroup M8a on Mitochondrial Energy Metabolism of Transmitochondrial Cybrids
Wen Chaowei, Qiu Ruyi, Hu Nianqi, Lü Jianxin*
School of Laboratory Medicine and Life Science, Wenzhou Medical University,Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou 325035, China
Abstract: To observe the effects of mitochondrial DNA (mtDNA) haplogroup M8a on mitochondrial energy metabolism， transmitochondrial cytoplasmic hybrid cells (cybrids) from haplogroups M8a and G2a were established in this study. mtDNA copy numbers and transcriptional levels were detected by Real-time qPCR. Reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were determined by Microplate Reader.Mitochondrial respiration was measured by Oxygraph-2k high-resolution respirometry. The results showed that mtDNA copy numbers and light-strand transcriptional levels remarkably decreased， basal respiration， respiration for ATP production， maximal respiration and MMP significantly decreased， while ROS production markedly increased in cybrid cells from mtDNA haplogroup M8a compared with haplogroup G2a cybrids. The results indicated that mtDNA haplogroup M8a might contribute to the risk of type 2 diabetes mellitus.
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