Molecular Cloning and Biochemical Function Prediction of A New Gene from Mouse

Abstract: In this study， a new gene coding putative nucleic acid binding protein from mouse was cloned. The gene expression pattern and its predicted protein function had also been discussed. By utilizing promoter-trap technology， a DNA fragment with promoter activity was obtained from mouse genomic DNA. A putative gene located in the downstream of this DNA fragment was predicted by bioinformatics analysis. The predicted gene was 1 504 bp in length with an open reading frame of 540 bp. The predicted open reading frame was confirmed by reverse transcription PCR (RT-PCR). Homology analysis with other species showed that the sequence of this gene shares similarities with the nucleic acid binding protein from Rattus norvegicus and RNA binding protein from Pan troglodytes of 78% and 40%， respectively. The gene expression properties in diverse tissue types under different physiological status or pathological status were studied by quantitative Real-time PCR (qRT-PCR). As in all the tissue types tested from mice bearing tumor， the expression levels of this gene are much higher than that in healthy mice. These results indicate that this new gene is possibly involved in tumorigenesis.

CSTR: 32200.14.cjcb.2017.05.0009