miR133a Down-Regulates the Expression of Gdnf Gene to Inhibit the Development of Ureteric Bud

Abstract: The present study predicted and testified that miR133a could regulate glial cell line derived neurotrophic factor (Gdnf)， a protein generated by metanephrogenic mesenchyme (MM)， which is of great importance in the development of ureteric bud (UB) and investigated inhibitory effect of miR133a towards the development of UB. Bioinformatics analysis displayed the high conservation of the Gdnf mRNA 3′ untranslated regions (3′UTR) between different species and predicted that miR133a could probably bind to it. The results of luciferase assay confirmed that miR133a could directly target Gdnf mRNA 3′UTR. The results of Real-time PCR and Western blot revealed that miR133a down-regulated the expression of Gdnf both at mRNA and protein levels.Furthermore， we found significant reduction in the ability of miR133a treated cells to contribute to ureteric bud formation in embryonic kidney rudiments culture system. Taken together， our results suggest that miR133a might inhibit the formation of ureteric bud through its binding to complementary sequences in the Gdnf mRNA 3′UTR.

CSTR: 32200.14.cjcb.2017.05.0007