Expression and Function of Mannose Receptor of Dendritic Cells Induced by Ovalbumin in WHBE Rabbits

Tu Jue¹, Gu Huanpeng², Cai Yueqin¹, Ling Yun¹, Liu Junping¹, Xu Jianqin^1*

¹Comparative Medical Institute, Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053,China;
²School of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China

Abstract: This paper aimed to study the expression and function of mannose receptor (MR) of dendritic cells (DCs) induced by ovalbumin (OVA) in white hair black eyes (WHBE) rabbits. The peripheral blood lymphomonocytes were separated and cultured for six days in RPMI 1640 complete medium with both 20 ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF) and 20 ng/mL interleukin-4 (IL-4)， and exhibited typical dendritic morphology. Besides， the levels of CD86 and human leukocyte antigen-DR (HLA-DR) on cell surface were detected by flow cytometry at six days of culture. The results showed that expressions of CD86 and HLA on cell surface were significantly upregulated， which defined typical DCs. The results of Real-time PCR assay showed that MR mRNA levels of DCs were both upregulated induced by OVA in two strains of rabbits， moreover，the MR mRNA levels in WHBE rabbits were higher than Japanese white (JW) rabbits. The results of OVA647 internalization detected by flow cytometry showed that allergen uptake of DCs reduced after stimulation by OVA and MR inhibitor could significantly down-regulate the antigen uptake. Meanwhile， OVA647 internalization of DCs in WHBE rabbits was higher than which in JW rabbits after equal treatments. In conclusion， it suggested that DCs in WHBE rabbits could active Th2 type T cells polarization， mediated by high levels of CD86 and MR with boost of antigen presentation， which subsequently lead to airway allergic inflammation. The sensitivity of DCs to allergen in WHBE rabbits may largely depend on the expression and function of mannose receptor.

CSTR: 32200.14.cjcb.2017.05.0006