Study on the Biological Characteristics of Rat Skeletal Muscle-Derived Satellite Cells from Rats in Different Age

Abstract: This study was aimed to study on the biological characteristics of rat skeletal muscle-derived satellite cells (MDSCs) in rats aged 5， 10， 15， 21， 42 days. MDSCs were separated and cultured by double enzyme digestion and differential adherent culture. Neurogenic， osteogenic and myogenic induction media were used to induce directed differentiation. Differentiated nerve cells， osteoblasts and myotubes cells were identified by morphology and immunohistochemical staining， respectively. Primary MDSCs of the 5th， 10th， 15th and 21st day were highly efficiently separate from homologous skeletal muscles with a wide range of source. It was difficult to separate the MDSCs from 42nd day rat. After neurogenesis， four groups MDSCs formed neuron-specific enolase positive olygonal-shaped dendritic cells. It is the first time to identified that MDSCs can be differentiated into neural cells.After osteogenesis， MDSCs in rats aged 5， 10， 15， 21， 42 days formed Alizarin red- and osteocalcin-positive bone nodules. After myogenesis， myotubes were formed and expressed fast muscle myosin-positive. MDSCs of the 5th，10th， 15th and 21st day are easy to isolate culture and amplify in vitro with pluripotency， which increases the range of source material available for tissue engineering and are suitable as seed cells for regenerative medicine.

CSTR: 32200.14.cjcb.2017.05.0005