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Preparation of SEs-Based Cationic Liposomes and Their Gene Delivery Performance
Liu Shengqiang, Zhao Yinan*, Li Mingshan, Liu Hongyu, Li Haiyue, Zhang Shubiao
College of Life Science, Dalian Nationalities University, Dalian 116600, China
Abstract: A quaternary ammonium-based lipid， and sucrose esters (SEs) as helper-lipids， were prepared into liposomes. The size， Zeta potential and morphology of cationic liposomes were researched. The sizes of these liposomes were from 210-230 nm， and Zeta potential were in the range of 50-65 mV. Lipoplexes formed by the cationic liposomes with pGFP-N2 (plasmid green fluorescent protein-N2) were evaluated in terms of gene transferring efficiency and cytotoxicity in Hep-2 and Hela cells. The liposomes with SEs as helper lipid showed high transfection at the lipid/SEs ratios of 1׃1 and 2׃1. Cytotoxicity assay indicated that SEs could reduce the toxicity of liposomes to some extent. The results convincingly demonstrated that SEs could be highly desirable candidates for gene delivery systems.
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