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The Impact and Mechanism of Downregulation of CD13 in Osteosarcoma on Tumor Angiogenesis
Chen Qianzhu1, Ma Lei1, Jiang Shifang, Huang Wei2, Li Longjiang1*
1Department of Pathophysiology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;
2Department of Orthopaedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
2Department of Orthopaedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract: Cluster of differentiation 13 (CD13) is able to accelerate the progression of tumor cells and promote tumor angiogenesis. In the present work， the expression of CD13 in osteosarcoma cell MG63 was reduced in order to investigate the interleukin-6 (IL-6) level released from MG63 and phosphorylation level of signal transducer and activator of transcriptionTyr705 (STAT3Tyr705) in vascular endothelial cells (HUVEC) by ELISA and Western blot. Moreover， the effects and mechanisms of CD13 expressed in MG63 on HUVEC migration， invasion， vascular lumen formation were also investigated.The results showed that downregulation of CD13 in osteosarcoma MG63 could inhibit IL-6 release and decrease the phosphorylation of STAT3Tyr705 in HUVEC， contributing to reducing HUVEC migration， invasion and angiogenesis. The exogenous IL-6 neutralized above effects involved in downreulated CD13 in MG63 cells. Inhibition of CD13-IL-6-STAT3 signaling pathway could impede tumor angiogenesis and became novel target for therapy of osteosarcoma.
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