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Determination of the Subcellular Localization of Proteins as An Experimental Course in Cell Biology
Song Jieqiong, Bao Shuguang, Li Luhua, Zhu Shifeng, Men Shuzhen*
Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Abstract: Genome sequencing has resulted in the identification of a large number of genes with unknown function. Determination of the subcellular localization of the encoded proteins is crucial for identifying their function. Transient expression is a simple and fast technique to study the subcellular localization of proteins.Here， we present an experimental course suitable for undergraduate students. In this course， students will analyze the intracellular localization of fluorescent proteins transiently expressed in tobacco leaves. We describe the experimental principle， step-by-step protocol， and teaching arrangement in detail. This experiment provides valuable references for the experimental teaching for undergraduate students.
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