Home > Browse Issues > Vol.39 No.3
Growth and Viral Infection of MRC-5 Cells in A Three-Dimensional Collagen Culture Model
He Bing, Chen Guomin*, Zeng Yi*
State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract: In this study， human embryonic lung fibroblasts (MRC-5) were cultured in the three-dimensional (3D) culture model with rat tail tendon collagen type I as a scaffold. The effects of conventional culture media and PDGF (platelet-derived growth factor) media on the growth status of MRC-5 cells were investigated in a 3D culture model. And the effects of the cellular growth status on the surface of 3D collagen matrices with two different culture media were analyzed. The infection of HCMV (human cytomegalovirus) was observed in the 3D culture model. The results showed that conventional culture media and PDGF media promoted cells to overlap each other and formed a multilayer three-dimensional network structure. PDGF media promoted cells to protrude dendritic extensions at early stage. Conventional culture media helped cells to keep multilayer network structure for longer time. Furthermore， MRC-5 cells in conventional culture media were contracted into clusters， whereas cell migration in PDGF media were observed as individual cells. HCMV could induce the cytopathic effect (CPE) of MRC-5 cells cultured in 3D collagen. It is possible that the 3D culture model of MRC-5 cells will contribute to cellular biology，cell morphology and viral infection.
CN
EN