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Expression of Oncostatin M in Kidney of MRL/lpr Mice
Zhang Wei1, Wang Hui1, Li Hongbo1, Du Yunxia1, Xing Lingling2, Xu Ning1, Liu Qingjuan1*
1Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China;
2Department of Nephrology, the Second Affiliate Hospital of Hebei Mdical University, Shijiazhuang 050000, China
2Department of Nephrology, the Second Affiliate Hospital of Hebei Mdical University, Shijiazhuang 050000, China
Abstract: The aim of this study is to investigate the expression of OSM (oncostatin M) in the renal cortex of MRL/lpr mice. MRL/MpJ mice were designated as the control group and MRL/lpr mice were designated as the lupus group. The expression of OSM was detected by the methods of immunohistochemistry and enzyme linked immunosorbent assay. Western blot and quantitative Real-time PCR (qRT-PCR) analysis were used to determine the protein and mRNA expression of E-cadherin， α-SMA (α-smooth muscle actin) and Col I (collagen I). qRTPCR analysis was used to detected the expression of fibronectin (FN) mRNA. The results suggested that the OSM expression increased in lupus mice compared with control mice. And the expression level of OSM is positively correlated with blood urea nitrogen， 24 h urinary protein and the expression level of α-SMA， Col I and FN but is negatively correlated with the expression level of E-cadherin. The results indicated that OSM expression is increased in kidney in lupus mice， which may connected with the renal dysfunction and tubular-epithelial cell transdifferentiation.
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