The Study on the Relationship between MYH9 and TRIF-GEFH1-RhoB Signaling Pathway in Dendritic Cells

Abstract: In order to explore the relationship between the ras homolog family member B (RhoB) targeting protein nonmuscle myosin heavy chain IIa (MYH9) and TRIF-GEFH1-RhoB signal pathway， the relationship between MYH9 and TIR domain-containing adapter inducing IFNβ (TRIF) pathway， guanine nucleotide-exchange factors H1 (GEFH1) and major histocompatibility complex II (MHCII) were investigated through Real-time quantitative PCR technique and siRNA interference technique， laser scanning confocal microscopy， flow cytometry and gene knockout mice. The results showed that lipopolysaccharide (LPS) induced the up-regulation of MYH9 mRNA level in DCs from wild-type (WT) mice but not in DCs from TRIF knockout， and siRNA of GEFH1 significantly suppressed the LPS-mediated up-regulation of MYH9 mRNA level (P<0.01). After LPS stimulation，MYH9 colocalized with MHCII in DCs. In addition， RNAi of MYH9 inhibited the LPS-mediated surface expression of MHCII in DCs (P<0.05). These results indicated that there was a correlation between MYH9 and TRIF-GEFH1-RhoB signaling pathway.

CSTR: 32200.14.cjcb.2017.03.0008