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The Induction of Apoptosis in Human Gastric Cancer SGC-7901 Cells by Pterostilbene
Feng Yunjianan, Shao Shuli*, Zhang Xirui, Xia Yan, Zhang Weiwei, Zhu Shaowei
College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
Abstract: Human gastric cancer SGC-7901 cells were used to observe the effect of pterostilbene on cells proliferation and cells apoptosis， and analysis the expression of bax (B-cell lymphoma-2 associated X) and bcl-2 (B-cell lymphoma-2)， which providing theoretical basis for mechanisms of apoptosis. MTT assay was used to determine SGC-7901 cells activity， fluorescence microscope were used to detect apoptosis， flow cytometry to detect cell apoptosis rate and cell cycle. The mRNA and protein levels of bax and bcl-2 were measured by qRT-PCR and Western blot with different concentrations of pterostilbene treating human gastric cancer SGC-7901 cells after 48 h.The results showed that the IC50 was 53.44 μg/mL， the cells treated with pterostilbene showed a typical apoptotic morphology， with the increase of drug concentration in the early apoptosis and late apoptosis percentage increased and the G1 arrest was induced significantly after treated with pterostilbene after the SGC-7901 cells treated with pterostilbene for 48 h in this study. The results suggested that pterostilbene inhibited the proliferation of SGC-7901 cells in a dose-dependent manner and induced the apoptosis of gastric cancer cells through up-regulation of bax and down-regulation of bcl-2.
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