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The Mechanism of MicroRNA-1 Regulating the Cell Cycle in Human Colon Cancer
Chen Tongke1#, Chen Linhua2#, Wang Jiao2, Wang Lihua2*
1Laboratory Animal Center, Wenzhou Medical University, Wenzhou 325000, China;
2School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027, China
2School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027, China
Abstract: microRNA-1 was transfected into human colon cancer cell line HT-29 and HCT 116. The proliferation of HT-29 and HCT 116 cells was examined by MTS cell proliferation assay and colony formation assay. The cell cycle was analyzed by Flow Cytometry. The results showed that miR-1 could inhibit HT-29 and HCT 116 cells proliferation， colony formation， and induce G1-phase cell cycle arrest， and suppress tumor growth in a xenograft mouse model. Furthermore， we identified CCND1 (cyclin D1) and CDK6 (cyclin dependent kinases 6) as the direct targets of miR-1 by dual-luciferase activity assay and Western blot. miR-1 also down-regulated the expression of CDK2， CDK4， phosphorylated-Rb (retinoblastoma gene)， E2F1 (E2F transcription factor 1)，phosphorylated-Cdc2 (cell division cycle 2) indirectly. Our findings suggested that miR-1 may function as a novel tumor suppressor in human colon cancer.
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