Deficiency of Paneth Cells Lysozyme in Small Intestine of Mice Infected by ToxoDB#17 Strain Toxoplasma gondii

Abstract: In order to investigate the small intestine pathological lesions and the change of Paneth cells (PCs) lysozyme after infection of ToxoDB#17 strain Toxoplasma gondii， 1×106 ToxoDB#17 strain T. gondii oocysts were fed to Kunming mice orally. Pathological lesions and the distribution of T. gondii in small intestine， Paneth cells and expression of lysozyme were observed on 6 hours after inoculation (HAI)， 1， 3 or 8 days after inoculation (DAI) by HE and immunohistochemical staining， respectively. The results showed that 100% of the mice fed with 1×106 oocysts were infected. The positive area of T. gondii in the intestine was increased with prolonged infection time (P<0.05)， but the lesions did not change significantly. The number of crypts， crypts containing PCs， PCs and granules in intestine were decreased firstly， then increased， decreased last. They were the highest at 3 DAI，compared to the control group (P>0.05). It was negative staining of lysozyme in granules of PCs after infection of T. gondii. These results indicated that ToxoDB#17 strain T. gondii infection caused the deficiency of the Paneth cells lysozyme in intestine of mice. The pathological lesions of ToxoDB#17 strain T. gondii on small intestine of mice were lightly. PCs and their granules had responded to T. gondii. The deficiency of lysozyme in PCs may have relation with succeed invasion intestine of T. gondii.

CSTR: 32200.14.cjcb.2017.02.0009