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microRNA-10a/b Regulate Neuron Development by Repressing Mib1 in Zebrafish
Lü Feng1,2, Shi Yunwei3, Wang Xin3, Liu Dong3*, Yan Xinghong1*
1College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2Nantong Science and Technology College, Nantong 226007, China;
3Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China
2Nantong Science and Technology College, Nantong 226007, China;
3Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China
Abstract: To study the role of miR-10a/b in neuronal development of zebrafish， firstly we investigated the expression of miR-10a/b in embryonic neural tube by whole-mount in situ hybridization and TaqMan PCR. Then we established zebrafish model with miR-10a/b knockdown using morpholino (Mo) oligonucleotide antisenses. Based on this model， we monitored the abnormal phenotype of neuron in neural tube， and further analyzed whether down-regulated mindbomb E3 ubiquitin protein ligase 1 (Mib1) rescue abnormal phenotype caused by miR-10a-Mo injection. We found miR-10a and miR-10b were highly expressed in the zebrafish neural tube at 24 hours post fertilization (hpf) and 48 hpf. Neurons in dorsal neural tube were obviously reduced by miR-10a/b knockdown. In addition， down-regulated Mib1 could partially rescue neuron phenotype defects induced by miR-10 down-regulation and mRNA level of Mib1 in miR-10-Mo injected embryos was apparently increased. These results indicated that miR-10a/b affected the development of neurons in zebrafish by repressing the Mib1.
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