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The Role of Fatty Acid Desaturase in Flax fad3b in Transgenic Mice Model
Wei Zhuying, Jian Lu, Yang Lei, Gao Yang, Wang Dong, Chen Chen, Zuo Yongchun, Li Guangpeng*
Research Center for Laboratory Animal Science, Inner Mongolia University, Huhott 010070, China
Abstract: The fatty acid desaturase 3 (fad3) played a pivotal role in convert n-6 polyunsaturated fatty acids (n-6 PUFAs) to n-3 PUFAs in plants. In this study， fad3b， a family member of fad3 from Linum usitatissimum Linn was tranfected into C2C12 myoblasts. The n-6 PUFAs were significant decrease， and the n-3 PUFAs were significant increase in the transfected cells. Fad3b transgenic mice were produced by pronuclear microinjection， and the relative expression of fad3b in tissue were analysized. The results showed that in compared to non-transgenic mice， highest mRNAs level of fad3b was detected in liver， and its lower levels in skeletal muscle， fat， brain and heart. In addition， there is a significant reduction of n-6/n-3 PUFAs ratios in skeletal muscle， brain and liver， but no difference in fat， ovary and testis between transgenic and non-transgenic mice. Altogether， fatty acid desaturase fad3b from Linum usitatissimum Linn could be expressed in mouse myoblasts and transgenic offspring， and play the role to convert n-6 PUFAs to n-3 PUFAs in mammalian tissues. These results confirmed that in compared to fat1， the function of fad3b was more concentrated， and fad3b transgenic mouse could be more appropriate model for fertility studies.
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