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The Effect of miRNA-181b-5p on Proliferation and Apoptosis of Human Gastric Cancer Cell HGC-27
Zhang Xiaotian1*, Chang Xinjian2#, Song Linlin3#
1Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China;
2Laboratory of Molecular Genetics, Fenyang Hospital of Shanxi Province, Fenyang 032200, China;
3Department of Clinical Laboratory, Fenyang Hospital of Shanxi Province, Fenyang 032200, China
2Laboratory of Molecular Genetics, Fenyang Hospital of Shanxi Province, Fenyang 032200, China;
3Department of Clinical Laboratory, Fenyang Hospital of Shanxi Province, Fenyang 032200, China
Abstract: The miRNA-181b-5p levels in serum of gastric cancer patients and gastric cancer cell HGC-27 were detected， and the effect of miRNA-181b-5p on proliferation and apoptosis of gastric cancer cell HGC-27 were investigated， which in order to explore the role of miRNA-181b-5p in the progress and development of gastric cancer.Real-time quantitative PCR (qRT-PCR) was performed to detect miRNA-181b-5p levels in serum of gastric cancer patients and healthy people， and in gastric cancer cell HGC-27 and gastric mucosal cell GES-1. The miRNA-181b-5p inhibitiors and inhibitors negative control was tranfected into gastric cancer cell HGC-27 by Lipofectamine 2000，respectively. Then miRNA-181b-5p was identified by qRT-PCR. The effect of miRNA-181b-5p on the proliferation of gastric cancer cell HGC-27 was detected by CCK-8 assay. The cell apoptosis and cell cycle distribution were analyzed by flow cytometry. The qRT-PCR result showed that the miRNA-181b-5p level in serum of gastric cancer was increased compared with the healthy people (P<0.05)， and was significantly increased in gastric cancer cell HGC-27 compared with gastric mucosal cell GES-1 (P<0.01). The miRNA-181b-5p level in gastric cancer cell was decreased after miRNA-181b-5p inhibitiors transfection compared with the inhibitors negative control (P<0.01). The result of CCK-8 assay showed that the proliferation of gastric cancer cell HGC-27 was significantly reduced while the miRNA-181b-5p level was reduced (P<0.01). The result of flow cytometry showed that the apoptosis of gastric cancer cell HGC-27 was significantly up-regulated after transfection (P<0.01)， and the proportion of S-stage cells was decreased as well as the proportion of G2/M-stage cells was increased. The proliferation of gastric cancer cell was inhibited after the tranfection of miRNA-181b-5p inhibitors. These results indicated that the serum level of miRNA-181b-5p may act as an molecular marker for early diagnosis of gastric cancer， and miRNA-181b-5p may act as oncogene in the progress and development of gastric cancer and serve as a new target for the molecular treatment of gastric cancer.
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