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The Establishment of the Detection Method of G-Quadruplex of DNA Structure in Mammalian Cells
Xiao Yingnan, Yuan Jinghua, Zhang Miaomiao, Wang Feng*
Department of Genetics, School of Basic Medical, Tianjin Medical University, Tianjin 300070, China
Abstract: Guanine-rich oligonucleotides folded into quadruple-stranded helical structures known as G-quadruplexs (G4)， which have been associated with genomic stability and the regulation of gene expression.However， to date， the methods of imaging G-quadruplexes in mammalian cells remain poorly defined. Here， we reported the generation and application of a structure-specific antibody (BG4) that was employed to visualize DNA G-quadruplex structures and telomeric G-quadruplex by immunofluorescence-fluorescence in situ hybridization (IF-FISH) in adherent cells with extracting and treating by RNase. This is contributing to research of G-quadruplex structure more accurately and intuitively in vivo， definituding the interaction of between G-quadruplex (telomeric G-quadruplex) and ligands， and consolidating the position of G-quadruplex structure in the areas of genetics，epigenetics and pathology.
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