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Primary Culture and Identification of Astrocytes from Cereral Cortex of Rhesus Macaque
Wang Jianbin1,2, Feng Min1, Liao Yun1, Wang Lichun1, Zhang Ying1, Fan Shengtao1, Li Qihan1*
1Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming, 650118, China;
2Institute of Pathogen Biology Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China
2Institute of Pathogen Biology Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China
Abstract: The aim of this study is to establish an effective method to culture and identify the astrocytes from cerebral cortex of rhesus macaque. A male 6-month-old neonatal rhesus macaque was used， and the gray matter of cortex was isolated under aseptic conditions. The tissue was fully minced and pipetted to dispersed cell suspension to culture. The oligodendrocytes and microglias were removed through thermostatic oscillation，and differential attachment method was used to remove fibroblasts. The purified astrocytes were identified by immunocytochemical staining and immunofluorescence with anti-glial fibrillary acidic protein (GFAP). The results showed that the purity of astrocytes of rhesus macaque exceeded 95%， and the morphology of these cells had the typical characteristics of astrocytes. Thus the method to culture highly purified astrocytes of rhesus macaque was established successfully， which would be an invaluable tool for studying the roles of astrocytes in physiological and pathological states， and the pathogenesis and development of central nervous system diseases in vitro.
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