Home > Browse Issues > Vol.39 No.1
The Effect of Amphiphysin on the Phagocytosis of Escherichia coli by Drosophila S2 Cells
Sun Baozhen, Zhu Fei*
College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
Abstract: The effect of amphiphysin (Amph) on the phagocytosis of Escherichia coli (E. coli) by Drosophila S2 cells was studied in this paper. The observation under electron microscopy showed that heat-inactivated E. coli was phagocytosed by S2 cells， and there were typical phagocytic bodies in the S2 cells. The phagocytic bodies were not found in the S2 cells treated with Amph-siRNA. After S2 cells phagocytosed E. coli， the mRNA level of Amph gene was significantly increased. However， the protein level of Amph in Amph-siRNA S2 cells was significantly lower than that of normal S2 cells and S2 cells which phagocytosed E. coli. These results indicated that Amph plays an important role in the process of phagocytosis of E. coli in Drosophila S2 cells.
CN
EN