Ikaros Regulates FUT4 Promoter Activity and Its Mechanism

Abstract: Fucosyltransferase IV (FUT4) is the key enzyme in the process of the fucosylation. It is involved in the proliferation， migration and infiltration of tumor cells， but the mechanism of transcription regulation of FUT4 remained unclear. In this study， we constructed different recombinant plasmids carrying FUT4 promoter segments with different lengths according to the bioinformatics analysis and identified their transcriptional activities in 293T cell line. It’s found that there were potential DNA binding sites in the promoter of FUT4 according to analyses of FUT4 promoter by ALGGEN online. This study aims to explain the regulation of FUT4 by Ikaros to supply the FUT4 regulation mechanism. The transcriptional activities were analyzed by double luciferase method. The effects of Ikaros on FUT4 transcriptional activity were analyzed. The results showed that 1.2 Kb upstream of the FUT4 transcription initiation site (FUT4-1.2 Kb) had the highest transcriptional activity in 293T cells. Ikaros inhibited the transcriptional activity of FUT4 in a dose-dependent manner. When Ikaros DNA-binding sites were deleted， it was possible to remove the inhibitory effect of Ikaros on FUT4 transcriptional activity.

CSTR: 32200.14.cjcb.2017.01.0005