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Restore Effect of Vitamin C on the Antioxidant Capacity of Erythrocytes in Aged Rats
Wang Yueming, Yu Zhenhai, Chen Zhongquan, Cui Qian, Zhang Hui,Yang Saisai, Gai Xinxin, Zhang Ya, Cui Yuying, Xiong Yanlian*
Department of Human Anatomy, College of Basic Medicine, Binzhou Medical University, Yantai 264000, China
Abstract: The main purpose of the present study was to investigate the effects of vitamins C supplements on the antioxidant capacity of erythrocytes obtained from young and aged rats. Rats were assigned based on age to four groups:young (Y) group， young vitamin C supplement group (Yc)， aged (A) group and aged vitamin C supplement group (Ac).The contents of GSH， GSSG， GSH/GSSG and TFG of red blood cells collected from different age stages were evaluated.L-cysteine transport and ferric-reducing antioxidant power (FRAP) value were studied. An in vitro oxidation system was constructed to explore the lipid peroxidation and methemoglobin levels. The results showed that compared with young group， the GSH content， TFG content and GSH/GSSG ratio decreased significantly， meanwhile， GSSG content increased significantly in aged group. In the aged group， vitamin C supplementation induced significantly increase in TFG content and significantly improved L-cysteine transport capacity and antioxidant capacity. Under oxidative conditions in vitro，the lipid peroxidation and methemoglobin levels and L-cysteine influx rate significantly decreased in vitamin C treatment group. We present evidence of an improvement in the antioxidant capacity of RBCs by enhancing the influx rate of L-cysteine through intraperitoneal injection of vitamin C in aged rats.
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