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Acteoside Protected Hepatocytes from H2O2-Induced Apoptosis by PI3K/Akt/GSK3β Pathway
Zhao Pei1, Ye Tingjie2, Yan Xiaofeng2, Wang Xiaoling2*
1The Public Experiment Platform, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
2Department of Biology, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2Department of Biology, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Abstract: In the present study， we investigated that acteoside inhibited the H2O2-induced apoptosis through PI3K/Akt/GSK3β signal pathway. H2O2 was used to induce injury of fetal hepatocyte from mice. Cell viability was determined by CCK-8 assay， and apoptosis was assayed by flow cytometry. Western blot was used to investigate protein levels of Bcl-xL， Bax， Cyt-c， Akt， p-Akt， GSK3β and p-GSK3β. Compared with H2O2， acteoside can increase the cell viability and decrease the numbers of apoptotic cells. Meanwhile， the ratio of Bax/Bcl-xL downregulated.The release of Cyt-c from mitochondria into cytoplasm inhibited. The phosphorylation of Akt and GSK3β enhanced. These results suggested that acteoside protected fetal hepatocytes of mice from H2O2-induced apoptosis by PI3K/Akt/GSK3β signal pathway.
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