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The Induction of Apoptosis in Human Gastric Cancer SGC-7901 Cells by Allicin
Zhu Shaowei, Shao Shuli*, Zhang Lei, Zhang Weiwei, Chen Lin, Liu Chang
College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161000, China
Abstract: This work was aimed to investigate the apoptosis-inducing effect of allicin in human gastric cancer SGC-7901 cells and analyze its molecular mechanisms. MTT assay was used to determine SGC-7901 cells activity. Morphological changes of cells were observed under optical and laser confocal microscope. Apoptosis rate and cell cycle distribution were detected by flow cytometry. The expressions of Bax and Bcl-2 were measured by qRT-PCR and Western blot. The results showed that the IC50 was 78 μg/mL after the SGC-7901 cells treated with allicin for 48 h in this study. The cells treated with allicin showed a typical apoptotic morphology. The apoptosis rate was up to (61.15±3.77)% and the G1 arrest was induced significantly after treated with allicin. The results suggested that allicin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner， and induced the apoptosis of gastric cancer cells through up-regulation of Bax and down-regulation of Bc1-2.
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