Effect of Acanthopanax senticosus Pretreatment on Acute Renal Ischemic Reperfusion Injury in Rats

Abstract: This work aimed to investigate the protective effect of Acanthopanax senticosus (AS) injection on rats with acute renal ischemic reperfusion injury (IRI) and its possible mechanism. In this study， rats with acute renal ischemia reperfusion injury model was constructed by single-artery occlusion. Puncture bladder for urinary to measure kidney injury molecule-1 (KIM-1)， 6-acetyl-B-D-glucosaminidase (NAG) by enzyme-linked immunosorbent-assay (ELISA). The levels of serum Scr， BUN， IL-6， MDA were measured， respectively. Pathological changes of renal tissues were observed by hematoxylin-eosin staining (HE) staining technique. The protein levels of intercellular adhesion molecule-1 (ICAM-1) in renal tissues were detected by immunohistochemical method. The results of HE staining showed that IRI group and AS group had a certain degree of pathological changes， while all these pathological changes relieved in AS group but found no significant changes in control group. In AS group， levels of serum Scr， BUN， MDA， IL-6， KIM-1 and NAG content of urinary， the protein levels of ICAM-1 in renal tissues were significantly lower compared with IRI group. After acute renal ischemic reperfusion injury in rats， AS pretreatment could reduced levels of serum Scr， BUN， MDA， IL-6， KIM-1 and NAG content of urinary， the protein levels of ICAM-1 in renal tissue. AS could protect the kidney against renal ischemia reperfusion injury. That might be realized by its inhibition of inflammation.

CSTR: 32200.14.cjcb.2016.12.0009