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Effect of Fuantai-03 on Invasion of Human Ovarian Cancer HO-8910PM Cells
Ming-Neng Liao, Hai-Ying Xu, Run-Di Ma*, Li-Jian Yu*, Wei-Ming Su, Lai-Zhen Huang, Xiao-Yu Zhang, Ting-Xi Yu*
Key Laboratory of Marine Materia Medica, Guangdong Ocean University, Zhanjiang 524025, China
Abstract: MTT assay was adapted to measure the effect of Fuantai-03 (FAT-03) isolated from Dasyatis akajei on the growth of human ovarian cancer HO-8910PM cells; artificial reconstituted basement membrane was utilized to evaluate the invasive activity; SDS-PAGE was employed to analyze the effects of FAT-03 on the activity and secretion of MMP-9 by HO-8910PM cells. The results showed that cell growth inhibition rates were <10% after the treatment of HO-8910PM with FAT-03 (20.0， 40.0， 60.0 mg/ml) for 24 h， and that FAT-03 (20.0， 40.0， 60.0 μg/ml) inhibited HO-8910PM cells to adhere to the basement membrane components FN and Matrigel by 15.1%， 28.0% (P<0.05)， 54.1% (P<0.01)， and 14.7%， 34.3% (P<0.05)， 40.5% (P<0.01)， respectively， and decreased the percentages of locomoting and migrating HO-8910PM cells by 16.4%， 33.8% (P<0.05)， 42.6% (P<0.01)， respectively. In addition， FAT-03 significantly inhibited the activity of MMP-9 and its secretion by HO-8910PM cells in a dose-dependant manner. These experimental facts suggest that FAT-03 can significantly inhibit the invasion of HO-8910PM cells.
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