Home > Browse Issues > Vol.38 No.12
Culture of Human Olfactory Mucosa Neural Stem Cells In Vitro and Effects of Fluoxetine on Their Proliferation and Neurogenesis
Xie Yangzhi1, Li Qianlu1, Yang Deyu1,2, Fang Liang2, Liao Juan2, Li Yuan2, Wang Juan1, Liu Li3, Jiang Hong4, Li Zhiwei1*
1Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
2Central Laboratory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
3Department of Health Management, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
4Department of Otolaryngolory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China
2Central Laboratory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
3Department of Health Management, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
4Department of Otolaryngolory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China
Abstract: Human olfactory neural stem cells are satisfactory materials apllied in neuropsychiatry diseases recently discovered. Antidepressants could promote proliferation and neurogenesis of neural stem cells in human.However， culture method in vitro and effects of antidepressants on their proliferation and neurogenesis remains unknown. In the present study， tissue piece culture method and neurosphere method were used to harvest olfactory cells and purify neural stem cells separately. Nestin immunofluorescence was performed to verify the specificity of neural stem cells. Neural basal medium was used to induce the differentiation into neuron from neural stem cells.TUJ1 and MAP2 immunofluorescence were performed to verify the specificity of neuron. CCK-8 and Western blot were applied to measure effects of antidepressants on proliferation and differentiation of neural stem cells. Results showed that olfactory neural stem cells aggregated as neurosphere. Immunofluoscence assay showed Nestin， TUJ1，MAP2 positive in the treated cells. Compared with control group， antidepressants enhance the proliferation of neural stem cells， also， the expression of TUJ1 were up-regulated. Antidepressants could increase neurogenesis of neural stem cells which was likely to be one of the mechanisms in antidepressant treatment.
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